Cheng-Prusoff: IC50 = Ki·(1 + [S]/Km); Ki from competitive IC50

Layer 3 — Biologyin the neuropharmacology subtree

Cheng & Prusoff (1973) derived the closed-form relation between the experimentally-measured IC50 of a competitive enzyme inhibitor (the inhibitor concentration producing 50% inhibition of a fixed-substrate assay) and the true thermodynamic…

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