Inhibitor binds the active site and competes with substrate. Apparent K_M increases by factor (1 + [I]/K_I); Vmax unchanged. Lineweaver-Burk lines intersect on the y-axis. Classic examples: methotrexate vs dihydrofolate on DHFR; statins vs…
Inhibitor binds the active site and competes with substrate. Apparent K_M increases by factor (1 + [I]/K_I); Vmax unchanged. Lineweaver-Burk lines intersect on the y-axis. Classic examples: methotrexate vs dihydrofolate on DHFR; statins vs…