iPSC differentiation protocols (Yamanaka-Takahashi)

Layer 3 — Biologyin the Tissue Engineering & Regenerative Medicine subtree

Yamanaka-Takahashi 2006: 4 transcription factors (OCT4/SOX2/KLF4/MYC) reprogram somatic cells to iPSC. Stage-specific differentiation cocktails recapitulate embryonic morphogen sequences (Wnt → Activin → BMP for endoderm; Wnt → BMP → SAG…

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