Linearisation of Michaelis-Menten: 1/v = (K_M/Vmax)·(1/[S]) + 1/Vmax. Historically used to read K_M from x-intercept (-1/K_M) and Vmax from y-intercept (1/Vmax). Biased estimator: the 1/v transform inflates variance at low [S], so weighted…
Linearisation of Michaelis-Menten: 1/v = (K_M/Vmax)·(1/[S]) + 1/Vmax. Historically used to read K_M from x-intercept (-1/K_M) and Vmax from y-intercept (1/Vmax). Biased estimator: the 1/v transform inflates variance at low [S], so weighted…