Perturb-seq pooled CRISPR + scRNA (Dixit-Regev)

Layer 3 — Biologyin the Single-Cell Biology subtree

Dixit-Parnas-Adamson-Regev 2016 / CROP-seq Datlinger 2017: pool of CRISPR-perturbed cells → scRNA-seq with sgRNA capture. Reads out functional consequences of 100s-1000s of gene knockouts in parallel. Enables genotype→phenotype mapping…

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