Transcriptome sequencing: fragment cDNA → sequence → align → count. Differential expression by DESeq2, edgeR (negative binomial). Applications: expression atlas, fusion detection, alt splicing, sc-transcriptomics.
Transcriptome sequencing: fragment cDNA → sequence → align → count. Differential expression by DESeq2, edgeR (negative binomial). Applications: expression atlas, fusion detection, alt splicing, sc-transcriptomics.