smFRET (Stryer-Haugland 1967)

Layer 2 — Chemistryin the biophysical-chemistry subtree

Stryer-Haugland 1967 FRET as spectroscopic ruler 1-10 nm; Ha-Selvin 1996 single-molecule FRET; modern smFRET with sCMOS + ALEX + 3-color decoding.

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