Tang-Surani 2009 first-mouse-blastomere scRNA-seq → Picelli 2013 Smart-seq2 full-length transcripts. Random-priming + template-switching + PCR-amplification on cDNA. ~6-10k genes detected per cell; superior coverage to droplet methods…
Tang-Surani 2009 first-mouse-blastomere scRNA-seq → Picelli 2013 Smart-seq2 full-length transcripts. Random-priming + template-switching + PCR-amplification on cDNA. ~6-10k genes detected per cell; superior coverage to droplet methods…