Activity-based probe first-order labeling framework: f(t) = 1 − exp(−k_obs·t) with half-life ln(2)/k

Activity-based probe (ABP) first-order irreversible labeling kinetics: a covalent probe reacts with an active-site residue (e.g. Cys, Ser nucleophiles) via pseudo-first-order kinetics when probe is in excess. The fraction of protein…