Activity-based protein profiling (Cravatt-Wright 2008) — a chemical-biology platform for measuring enzyme engagement in native cellular context. Probe architecture: a *warhead* that forms a covalent bond to the active-site residue of a…
Activity-based probe: warhead + linker + reporter; Langmuir occupancy
Related concepts
- Langmuir adsorption isotherm
- ABP Langmuir: θ=L/(K_d+L); θ(K_d)=1/2, θ(∞)=1, θ(0)=0
- PROTAC ternary-complex cooperativity framework: α = K_d^binary / K_d^ternary (E3-target-degrader design metric)
- Activity-based probe first-order labeling framework: f(t) = 1 − exp(−k_obs·t) with half-life ln(2)/k
- Bayes' theorem
- Activity-based probe