CRISPR-Cas9: sgRNA-directed DNA cleavage; 20-nt protospacer + 5'-NGG-3' PAM

CRISPR-Cas9 (Jinek et al. 2012, Cong et al. 2013, Mali et al. 2013, Doudna & Charpentier Nobel 2020) is the reprogrammable RNA-guided endonuclease system adapted from the Streptococcus pyogenes Type II-A CRISPR adaptive-immunity locus. …