CRISPR-Cas9

Layer 3 — Biologyin the Synthetic Biology subtree

Bacterial adaptive-immunity endonuclease repurposed for programmable genome editing. sgRNA guides Cas9 to target; DSB → NHEJ/HR. Jinek-Charpentier-Doudna 2012; Zhang + Church 2013 (mammalian). Nobel 2020.

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