Classical transition-state theory (Eyring 1935) predicts a maximum H/D kinetic-isotope-effect of KIE = k_H/k_D ≈ 6.9 at 298 K, set by the 3000 cm⁻¹ C–H vs 2200 cm⁻¹ C–D zero-point-energy difference. Measured enzymatic KIEs dramatically…
Classical transition-state theory (Eyring 1935) predicts a maximum H/D kinetic-isotope-effect of KIE = k_H/k_D ≈ 6.9 at 298 K, set by the 3000 cm⁻¹ C–H vs 2200 cm⁻¹ C–D zero-point-energy difference. Measured enzymatic KIEs dramatically…