Hill full framework: θ(L) = Lⁿ/(Kⁿ + Lⁿ); half-saturation at L = K; Hill slope n/4 at L = K

The Hill (1910) cooperative-binding equation in its fractional-occupancy form, θ(L) = Lⁿ/(Kⁿ + Lⁿ), where L is the free-ligand concentration, K is the half-saturation constant, and n is the Hill coefficient (1 = non-cooperative, >1 =…