Michaelis-Menten saturation kinetics (Michaelis & Menten 1913; Briggs-Haldane 1925 steady-state generalisation). For the single-substrate reaction E + S ⇌_{k₁,k₋₁} ES →_{kcat} E + P, steady-state d[ES]/dt = 0 gives [ES] = [E]_tot·S/(K_m +…
Michaelis-Menten v = V·S/(Km+S): steady-state ES kinetics
Related concepts
- Catalysis
- Chemical equilibrium
- v = V·S/(Km+S) at S = Km ⇒ v = V/2 (half-saturation anchor)
- 1/v = Km/(V·S) + 1/V; y-intercept 1/V, x-intercept −1/Km
- Competitive inhibition framework: K_m^app = K_m·(1 + I/K_i); V_max unchanged
- Eyring 1935: k(T) = (k_B T/h)·exp(−ΔG‡/RT)
- Sliding-filament mechanism
- Pharmacodynamics