Force-spectroscopy and fluorescence techniques on individual biomolecules — optical/magnetic tweezers, AFM pulling, FRET. Resolves piconewton forces and nanometre extensions, exposing the stochastic step-by-step kinetics that ensemble…
Single-molecule biophysics
Related concepts
- Reduced Planck constant (ℏ)
- Gaussian (normal) distribution
- Worm-like chain model
- Molecular motors (physics)
- Fluorescence correlation spectroscopy (FCS)
- Langevin equation (biophysical use)
- PROTAC cooperativity: α = 1 at neutrality; α = 10 at 10-fold positive cooperativity (canonical pins)
- Hill: θ = Lⁿ/(Kⁿ+Lⁿ); θ(L=K) = 1/2; slope dθ/d(log L)|_K = n/4